Journal: Bioactive Carbohydrates and Dietary Fibre
Article Title: Resistant starch from a tuberous root from the Andes cordillera improves metabolic and immune parameters in broilers.
doi: 10.1016/j.bcdf.2024.100420
Figure Lengend Snippet: Fig. 3. CD3+, CD4+ and CD8+ intestinal T lymphocytes subpopulations in broilers on day 35 determined by flow cytometry (upper left image). The images A, B, C correspond to dot plot made in the FlowJo XV software based on readings made in the Facs Canto II flow cytometer (Becton Dickinson). The images show: A. Distribution by size (FSC-A) and complexity (SSC-A) of the events analyzed in broilers, B. Percentage of CD4+ T cells in broiler chickens fed with a control diet C. Percentage of CD4+ T cells in broiler chickens fed with a diet supplemented with RS2.
Article Snippet: The labeling mix 2 was prepared with 2 μL of each of the following anti-chicken monoclonal antibodies: anti-CD3 epsilon (PC3/188A), antiCD8 alpha (EP72), the anti-human antibody anti-MHC1 (F21-2) coupled to FITC (Novus biologicals, Colorado, USA), which was used as a positive control, and 94 μL of cytometer buffer.
Techniques: Flow Cytometry, Software, Control